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KMID : 0364820100460040401
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Korean Journal of Microbiology
2010 Volume.46 No. 4 p.401 ~ p.404
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Effects of spTho1 Deletion and Over-Expression on mRNA Export in Fission Yeast
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Cho Ye-Seul
Yoon Jin-Ho
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Abstract
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Tho1 is a RNA-binding protein that assembles co-transcriptionally onto the nascent mRNA and is thought to be involved in mRNP biogenesis and mature mRNA export to cytoplasm in budding yeast. In fission yeast Schizosaccharomyces pombe, a homologue of THO1 (spTho1) was identified based on sequence alignment. A deletion mutant in a diploid strain was constructed by replacing one of spTho1-coding region with an ura4+ gene using one-step gene disruption method. Tetrad analysis showed that the spTho1 was not essential for growth. The spTho1 null mutant did not show any defects of bulk mRNA export. However, over-expression of spTho1 from strong nmt1 promoter caused the growth defects and accumulation of poly(A)+ RNA in the nucleus. These results suggest that spTho1 is involved in mRNA export from the nucleus to cytoplasm though it is not essential.
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KEYWORD
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knock-out mutant, mRNA export, over-expression, S. pombe, spThp1 gene
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